apob elisa kit  (R&D Systems)

Journal: Materials Futures

Article Title: A clinically defined and xeno-free hydrogel system for regenerative medicine

doi: 10.1088/2752-5724/ae4e4d

Figure Lengend Snippet: Substrate characterisation in 2D and 3D cell culture—biological responses and physical properties with different compositions. (a) hPSC confluence in 2D growth factor-reduced Matrigel (Geltrex), laminin 521, fibrin-laminin hydrogel, and fibrin gel after 4 d; scale bar = 100 μ m. (b) hPSCs cultured in 3D hydrogels. Top panel: fibrin (5 mg ml −1 ) gel. Bottom panel: Alphagel containing structures resembling pluripotent spheroids; scale bar = 200 μ m. Scanning electron microscopy of (c) fibrin gel and (d) Alphagel; scale bar = 1 μ m, magnification 20 K X, iProbe = 13 pA, 2.00 kV, Working Distancee = 4.4 mm for both images. (e) Young’s moduli in hydrogels with varying fibrin and laminin concentrations. One-way ANOVA; ** = p < 0.01, *** = p < 0.001, and **** = p < 0.0001. (f) Cell viability of hPSCs cultured in Alphagel, fibrin-only hydrogels, and 2D standard substrates. Mean ± sandard error of the mean displayed. t -test; * = p < 0.05. (g) ELISA of laminin 521 in culture media used with acellular Alphagel and (h) the calculated amount of fibrin-bound laminin.

Article Snippet: Apolipoprotein B (APOB) secretion in the supernatant was quantified using the human APOB ELISA quantification kit (Mabtech, no. 3715-1H-6) according to the product literature.

Techniques: Cell Culture, Electron Microscopy, Enzyme-linked Immunosorbent Assay

Journal: Materials Futures

Article Title: A clinically defined and xeno-free hydrogel system for regenerative medicine

doi: 10.1088/2752-5724/ae4e4d

Figure Lengend Snippet: Characterisation of iHeps derived in Alphagel, fibrin-only hydrogels, and Matrigel. (a) Key hepatocyte markers in Alphagel-derived iHeps. ALB = albumin, HNF = hepatocyte nuclear factor, CYP2A6 = Cytochrome P450 2A6, CD147 = cluster of differentiation protein 147, and E-CAD = E-cadherin. Scale bar = 25 μ m. (b) Key hepatocyte markers by gene expression (qPCR): CCAAT/enhancer-binding protein alpha (CEBPA), T-box transcription factor 3= TBX3, alpha-fetoprotein = AFP. (c) Albumin secretion (ELISA) and (d) CYP3A4 activity (P450-Glo TM ) in PHHs versus iHeps cultured in various gels (day 22). HCM = Hepatocyte Culture Media (Lonza). (e) LDL uptake (red) in Alphagel-derived iHeps versus hPSCs. Scale bar = 100 μ m. (f) CDFDA secretion (green) in Alphagel-derived iHeps versus hPSCs. Top panel scale bar = 20 μ m; bottom panel scale bar = 50 μ m. One-way ANOVA was used; * = p < 0.05, ** = p < 0.01, *** = p < 0.001, and **** = p < 0.0001.

Article Snippet: Apolipoprotein B (APOB) secretion in the supernatant was quantified using the human APOB ELISA quantification kit (Mabtech, no. 3715-1H-6) according to the product literature.

Techniques: Derivative Assay, Gene Expression, Binding Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Cell Culture

Journal: Materials Futures

Article Title: A clinically defined and xeno-free hydrogel system for regenerative medicine

doi: 10.1088/2752-5724/ae4e4d

Figure Lengend Snippet: Characterisation of iHeps cultured in Hepatogel and its effect on cell retention after intra-hepatic cell transplantation. (a) Differentially expressed genes in iHeps: Hepatologel, Alphagel, Matrigel, and adult PHHs. (b) A heat map summarising the differential gene expression of a hepatic 24-gene panel across replicates of Matrigel, Alphagel, and Hepatogel (normalised to hPSC). (c) Albumin ELISA of culture media and (d) luciferin-based measure of CYP3A4 activity: 2 d after completion of iHep differentiation and 2 d after plating PHHs. One-way ANOVA; * = p < 0.05, ** = p < 0.01, *** = p < 0.001, and **** = p < 0.0001. (e) Mouse livers 3 d after intra-hepatic injection with H-iHeps in Hepatogel and 0.9% saline. Red box = area magnified. * = site of injection, dotted white lines demarcate engrafted cell mass. Scale bar = 1 mm. (f) Human albumin (stained red) in engrafted iHeps 3 d after intra-hepatic injection. Scale bar = 100 μ m. (g) H-iHeps identified by albumin staining on liver histology 3 d after intra-hepatic injection. (h) ELISA of mouse serum for human albumin after injection with H-iHeps in Hepatogel and 0.9% saline over time. Day 0 = serum levels before injection. T -test; *** = p < 0.001 and **** = p < 0.0001.

Article Snippet: Apolipoprotein B (APOB) secretion in the supernatant was quantified using the human APOB ELISA quantification kit (Mabtech, no. 3715-1H-6) according to the product literature.

Techniques: Cell Culture, Transplantation Assay, Gene Expression, Enzyme-linked Immunosorbent Assay, Activity Assay, Injection, Saline, Staining

Journal: bioRxiv

Article Title: VDisk: Microfluidic Cartridge for Multimodal High-Yield, High-Purity Isolation of Extracellular Vesicles from up to 1 mL of Plasma

doi: 10.1101/2025.09.04.674190

Figure Lengend Snippet:

Article Snippet: Similarly, (V)LDL concentrations were measured using ApoB ELISA kit (R&D Systems, Catalog number DAPB00).

Techniques: Isolation

Journal: bioRxiv

Article Title: VDisk: Microfluidic Cartridge for Multimodal High-Yield, High-Purity Isolation of Extracellular Vesicles from up to 1 mL of Plasma

doi: 10.1101/2025.09.04.674190

Figure Lengend Snippet: Comparison of contaminant removal efficiency and particle size distribution among VDisk_0.22µm, VDisk_1.0µm, and SEC EV isolates from fasting and postprandial plasma. (A) Total protein removal, (B) ApoA1 removal representing HDL, and (C) ApoB removal representing (V)LDL particles. Each bar represents the mean of biological replicates (n = 3 healthy donors), and each data point corresponds to the average of three technical replicates. (D) Total particle concentration measured by NTA. Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test (n = 3). Significance levels: ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001. (E) And (F) EV size distribution profiles measured by NTA for different methods in fasting and postprandial conditions, respectively.

Article Snippet: Similarly, (V)LDL concentrations were measured using ApoB ELISA kit (R&D Systems, Catalog number DAPB00).

Techniques: Comparison, Clinical Proteomics, Concentration Assay

Journal: bioRxiv

Article Title: VDisk: Microfluidic Cartridge for Multimodal High-Yield, High-Purity Isolation of Extracellular Vesicles from up to 1 mL of Plasma

doi: 10.1101/2025.09.04.674190

Figure Lengend Snippet: Comparison of VDisk_1.0µm performance based on sample volume vs SEC. (A) EV isolation yield assessed by normalized TRF signal for CD81, (B) CD81 TRF signal per total protein, (C) CD81 TRF signal per HDL-associated ApoA, (D) CD81 TRF signal per (V)LDL associated ApoB, (E) CD81 positive EV percentage measured by nanoFCM, (F) total filtration time. Bars represent the mean of biological triplicates (n = 3 healthy donors); each point denotes an individual replicate. Statistical comparisons were performed using two-way ANOVA with Tukey’s multiple comparisons test. Significance levels: *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Similarly, (V)LDL concentrations were measured using ApoB ELISA kit (R&D Systems, Catalog number DAPB00).

Techniques: Comparison, Isolation, Filtration

Journal: The Journal of General Virology

Article Title: Hepatitis B virus is a stealth virus that minimizes proteomic and secretomic changes in primary human hepatocytes

doi: 10.1099/jgv.0.002170

Figure Lengend Snippet: Quantification of APOB in culture supernatants of HBV-infected PHH. PHHs were infected with HBV at an MOI of 1,000 VGE/cell, and culture supernatants were collected after the final 3 days of incubation (8 dpi). APOB levels were measured by ELISA. Data represent mean± sd from a single experiment; individual points represent four replicates.

Article Snippet: Secreted apolipoprotein B (APOB) levels in culture supernatants were measured using an ELISA kit (#KE00158-96T, Proteintech).

Techniques: Infection, Incubation, Enzyme-linked Immunosorbent Assay

Journal: Nature Metabolism

Article Title: Global 13 C tracing and metabolic flux analysis of intact human liver tissue ex vivo

doi: 10.1038/s42255-024-01119-3

Figure Lengend Snippet: a , Schematic of liver tissue sampling, culture and analysis. RNA-seq, RNA sequencing. b , Representative histology images of freshly resected (left) and 24-h cultured human liver tissue (right), from a total of 12 images. pv, portal vein; ha, hepatic artery; bd, bile duct. Scale bars, 250 µm. c , ATP content of freshly resected and 24-h cultured liver slices. d , ATP/ADP content, as in c . e , relative abundance of indicated metabolites in liver tissue and conditioned medium. g6p, glucose-6-phosphatase. f , Liver tissue synthesis rate (left scale) and plasma concentration (right scale) of albumin. Shaded area indicates the range of in vivo synthesis rate from previous reports (Supplementary Table ). g , Synthesis rate of APOB. h , Synthesis rate of triacylglycerides (TAGs). i , Synthesis rate of urea. Data from n = 3 independent tissue slices are shown for each donor in c – i .

Article Snippet: Apolipoprotein B was quantified by using the human APOB ELISA kit (R&D Systems, DAPB00, undiluted and diluted at 1:1,000).

Techniques: Sampling, RNA Sequencing, Cell Culture, Clinical Proteomics, Concentration Assay, In Vivo